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175489 syka c024  (Addgene inc)


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    Addgene inc 175489 syka c024
    175489 Syka C024, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/175489+syka+c024/Myc-Foxo1+LAALL+(pCMV5)+(Plasmid+%2317548)/pm39442513-239-31-30
    Average 92 stars, based on 1 article reviews
    175489 syka c024 - by Bioz Stars, 2026-10
    92/100 stars

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    Recombinant:

    Article Title: The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Tb-conjugated anti-6His antibody Cisbio/Revvity 61HISTLA; RRID: AB_2716834 Bacterial and virus strains BL21(DE3)-R3 pRARE CmR Addgene Addgene_26242 DH10Bac ThermoFisher Cat# 10361012 Chemicals, peptides, and recombinant proteins Sypro Orange Thermofisher S6650 cOmplete EDTA-free protease inhibitor cocktail Sigma Aldrich Cat# 5056489001 PhosphoSens peptide AssayQuant AQT0794 FCER1G pITAM peptide (residues 62–81): DGV(pY)TGLSTRNQET(pY)ETLKH (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107): ESP(pY)QELQGQRSDV(pY)SDLNT (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176): DQL(pY)QPLKDREDDQ(pY)SHLQG (LifeTein) This paper N/A FCER1G pITAM peptide (residues 62–81)-FITC: DGV(pY)TGLSTRNQET(pY)ETLKH-FITC (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107)-FITC: ESP(pY)QELQGQRSDV(pY)SDLNT-FITC (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176)-FITC: DQL(pY)QPLKDREDDQ(pY)SHLQG-FITC (LifeTein) This paper N/A CD79A pITAM peptide (residues 185–204)-FITC: ENL(pY)EGLNLDDCSM(pY)EDISRK-FITC (LifeTein) This paper N/A FCER1G non-phopho-ITAM peptide (residues 62–81): DGVYTGLSTRNQETYETLKH (LifeTein) This paper N/A TYROBP non-phopho-ITAM peptide (residues 88–107): ESPYQELQGQRSDVYSDLNT (LifeTein) This paper N/A CD3G non-phopho-ITAM peptide (residues 157–176): DQLYQPLKDREDDQYSHLQG (LifeTein) This paper N/A CD79A non-phopho-ITAM peptide (residues 185–204): ENLYEGLNLDDCSMYEDISRK (LifeTein) This paper N/A Deposited data Structure of the ITAM-free tSH2 domains of SYK This paper PDB: 7Q63 Structure of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5T Structure of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5U Structure of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5W SAXS data of the ITAM-free tSH2 domains of SYK This paper SASBDB: SASDUT7 SAXS data of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUU7 SAXS data of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUV7 SAXS data of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUW7 Experimental models: Cell lines Sf9 ThermoFisher Cat# 11496015 (Continued on next page) e1 Structure 32, 1–15.e1–e4, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Olilgonucleotides See Table S2 This paper N/A Recombinant DNA pNIC28-Bsa4 Addgene Addgene_26103 pFastBac1 ThermoFisher Cat# 10360014 SYKA-c020 (WT; tSH2 domains: aa 7–269) Bashore et al.8 Addgene_ 175489 SYKA-c024 (D249A; tSH2 domains: aa 7–269) This paper N/A SYKA-c025 (R197A; tSH2 domains: aa 7–269) This paper N/A SYKA-c026 (R195A; tSH2 domains: aa 7–269) This paper N/A SYKA-c027 (R175A; tSH2 domains: aa 7–269) This paper N/A SYKA-c028 (R22A; tSH2 domains: aa 7–269) This paper N/A SYKA-c029 (K232A; tSH2 domains: aa 7–269) This paper N/A SYKA-c030 (R45A; tSH2 domains: aa 7–269) This paper N/A SYKA-c031 (E242A; tSH2 domains: aa 7–269) This paper N/A SYKA-c032 (K247A; tSH2 domains: aa 7–269) This paper N/A SYKA-c033 (R42A; tSH2 domains: aa 7–269) This paper N/A SYKA-c034 (N19A; tSH2 domains: aa 7–269) This paper N/A SYKA-c036 (full length) This paper N/A Software and algorithms DALI Holm and Sander24 http://ekhidna2.biocenter.helsinki.fi/dali/ DIALS Winter et al.36 https://dials.diamond.ac.uk AIMLESS Evans and Murshudov37 https://ccp4.ac.uk PHASER McCoy38 https://ccp4.ac.uk REFMAC5 Murshudov39 https://ccp4.ac.uk MolProbity Williams et al.40 http://molprobity.biochem.duke.edu PyMol (v2.2.2) Schrödinger, Inc. https://www.pymol.org/ DynDom Hayward and Berendsen41 https://dyndom.cmp.uea.ac.uk/dyndom/ PDBePISA Krissinel and Henrick42 https://www.ebi.ac.uk/pdbe/pisa/ ScÅtterIV – www.bioisis.net FIDA software v2.37 Fida Biosystems – HullRad v9 Fleming and Fleming43 http://52.14.70.9/ Microsoft Excel v2404 Microsoft https://www.microsoft.com/ GraphPad Prism v10.2.3 GraphPad Software https://www.graphpad.com/ Other Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S ..

    Software:

    Article Title: The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Tb-conjugated anti-6His antibody Cisbio/Revvity 61HISTLA; RRID: AB_2716834 Bacterial and virus strains BL21(DE3)-R3 pRARE CmR Addgene Addgene_26242 DH10Bac ThermoFisher Cat# 10361012 Chemicals, peptides, and recombinant proteins Sypro Orange Thermofisher S6650 cOmplete EDTA-free protease inhibitor cocktail Sigma Aldrich Cat# 5056489001 PhosphoSens peptide AssayQuant AQT0794 FCER1G pITAM peptide (residues 62–81): DGV(pY)TGLSTRNQET(pY)ETLKH (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107): ESP(pY)QELQGQRSDV(pY)SDLNT (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176): DQL(pY)QPLKDREDDQ(pY)SHLQG (LifeTein) This paper N/A FCER1G pITAM peptide (residues 62–81)-FITC: DGV(pY)TGLSTRNQET(pY)ETLKH-FITC (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107)-FITC: ESP(pY)QELQGQRSDV(pY)SDLNT-FITC (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176)-FITC: DQL(pY)QPLKDREDDQ(pY)SHLQG-FITC (LifeTein) This paper N/A CD79A pITAM peptide (residues 185–204)-FITC: ENL(pY)EGLNLDDCSM(pY)EDISRK-FITC (LifeTein) This paper N/A FCER1G non-phopho-ITAM peptide (residues 62–81): DGVYTGLSTRNQETYETLKH (LifeTein) This paper N/A TYROBP non-phopho-ITAM peptide (residues 88–107): ESPYQELQGQRSDVYSDLNT (LifeTein) This paper N/A CD3G non-phopho-ITAM peptide (residues 157–176): DQLYQPLKDREDDQYSHLQG (LifeTein) This paper N/A CD79A non-phopho-ITAM peptide (residues 185–204): ENLYEGLNLDDCSMYEDISRK (LifeTein) This paper N/A Deposited data Structure of the ITAM-free tSH2 domains of SYK This paper PDB: 7Q63 Structure of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5T Structure of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5U Structure of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5W SAXS data of the ITAM-free tSH2 domains of SYK This paper SASBDB: SASDUT7 SAXS data of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUU7 SAXS data of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUV7 SAXS data of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUW7 Experimental models: Cell lines Sf9 ThermoFisher Cat# 11496015 (Continued on next page) e1 Structure 32, 1–15.e1–e4, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Olilgonucleotides See Table S2 This paper N/A Recombinant DNA pNIC28-Bsa4 Addgene Addgene_26103 pFastBac1 ThermoFisher Cat# 10360014 SYKA-c020 (WT; tSH2 domains: aa 7–269) Bashore et al.8 Addgene_ 175489 SYKA-c024 (D249A; tSH2 domains: aa 7–269) This paper N/A SYKA-c025 (R197A; tSH2 domains: aa 7–269) This paper N/A SYKA-c026 (R195A; tSH2 domains: aa 7–269) This paper N/A SYKA-c027 (R175A; tSH2 domains: aa 7–269) This paper N/A SYKA-c028 (R22A; tSH2 domains: aa 7–269) This paper N/A SYKA-c029 (K232A; tSH2 domains: aa 7–269) This paper N/A SYKA-c030 (R45A; tSH2 domains: aa 7–269) This paper N/A SYKA-c031 (E242A; tSH2 domains: aa 7–269) This paper N/A SYKA-c032 (K247A; tSH2 domains: aa 7–269) This paper N/A SYKA-c033 (R42A; tSH2 domains: aa 7–269) This paper N/A SYKA-c034 (N19A; tSH2 domains: aa 7–269) This paper N/A SYKA-c036 (full length) This paper N/A Software and algorithms DALI Holm and Sander24 http://ekhidna2.biocenter.helsinki.fi/dali/ DIALS Winter et al.36 https://dials.diamond.ac.uk AIMLESS Evans and Murshudov37 https://ccp4.ac.uk PHASER McCoy38 https://ccp4.ac.uk REFMAC5 Murshudov39 https://ccp4.ac.uk MolProbity Williams et al.40 http://molprobity.biochem.duke.edu PyMol (v2.2.2) Schrödinger, Inc. https://www.pymol.org/ DynDom Hayward and Berendsen41 https://dyndom.cmp.uea.ac.uk/dyndom/ PDBePISA Krissinel and Henrick42 https://www.ebi.ac.uk/pdbe/pisa/ ScÅtterIV – www.bioisis.net FIDA software v2.37 Fida Biosystems – HullRad v9 Fleming and Fleming43 http://52.14.70.9/ Microsoft Excel v2404 Microsoft https://www.microsoft.com/ GraphPad Prism v10.2.3 GraphPad Software https://www.graphpad.com/ Other Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S ..

    Mutagenesis:

    Article Title: The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Tb-conjugated anti-6His antibody Cisbio/Revvity 61HISTLA; RRID: AB_2716834 Bacterial and virus strains BL21(DE3)-R3 pRARE CmR Addgene Addgene_26242 DH10Bac ThermoFisher Cat# 10361012 Chemicals, peptides, and recombinant proteins Sypro Orange Thermofisher S6650 cOmplete EDTA-free protease inhibitor cocktail Sigma Aldrich Cat# 5056489001 PhosphoSens peptide AssayQuant AQT0794 FCER1G pITAM peptide (residues 62–81): DGV(pY)TGLSTRNQET(pY)ETLKH (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107): ESP(pY)QELQGQRSDV(pY)SDLNT (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176): DQL(pY)QPLKDREDDQ(pY)SHLQG (LifeTein) This paper N/A FCER1G pITAM peptide (residues 62–81)-FITC: DGV(pY)TGLSTRNQET(pY)ETLKH-FITC (LifeTein) This paper N/A TYROBP pITAM peptide (residues 88–107)-FITC: ESP(pY)QELQGQRSDV(pY)SDLNT-FITC (LifeTein) This paper N/A CD3G pITAM peptide (residues 157–176)-FITC: DQL(pY)QPLKDREDDQ(pY)SHLQG-FITC (LifeTein) This paper N/A CD79A pITAM peptide (residues 185–204)-FITC: ENL(pY)EGLNLDDCSM(pY)EDISRK-FITC (LifeTein) This paper N/A FCER1G non-phopho-ITAM peptide (residues 62–81): DGVYTGLSTRNQETYETLKH (LifeTein) This paper N/A TYROBP non-phopho-ITAM peptide (residues 88–107): ESPYQELQGQRSDVYSDLNT (LifeTein) This paper N/A CD3G non-phopho-ITAM peptide (residues 157–176): DQLYQPLKDREDDQYSHLQG (LifeTein) This paper N/A CD79A non-phopho-ITAM peptide (residues 185–204): ENLYEGLNLDDCSMYEDISRK (LifeTein) This paper N/A Deposited data Structure of the ITAM-free tSH2 domains of SYK This paper PDB: 7Q63 Structure of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5T Structure of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5U Structure of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper PDB: 7Q5W SAXS data of the ITAM-free tSH2 domains of SYK This paper SASBDB: SASDUT7 SAXS data of the FCER1G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUU7 SAXS data of the CD3G phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUV7 SAXS data of the TYROBP phospho-ITAM bound tSH2 domains of SYK This paper SASBDB: SASDUW7 Experimental models: Cell lines Sf9 ThermoFisher Cat# 11496015 (Continued on next page) e1 Structure 32, 1–15.e1–e4, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Olilgonucleotides See Table S2 This paper N/A Recombinant DNA pNIC28-Bsa4 Addgene Addgene_26103 pFastBac1 ThermoFisher Cat# 10360014 SYKA-c020 (WT; tSH2 domains: aa 7–269) Bashore et al.8 Addgene_ 175489 SYKA-c024 (D249A; tSH2 domains: aa 7–269) This paper N/A SYKA-c025 (R197A; tSH2 domains: aa 7–269) This paper N/A SYKA-c026 (R195A; tSH2 domains: aa 7–269) This paper N/A SYKA-c027 (R175A; tSH2 domains: aa 7–269) This paper N/A SYKA-c028 (R22A; tSH2 domains: aa 7–269) This paper N/A SYKA-c029 (K232A; tSH2 domains: aa 7–269) This paper N/A SYKA-c030 (R45A; tSH2 domains: aa 7–269) This paper N/A SYKA-c031 (E242A; tSH2 domains: aa 7–269) This paper N/A SYKA-c032 (K247A; tSH2 domains: aa 7–269) This paper N/A SYKA-c033 (R42A; tSH2 domains: aa 7–269) This paper N/A SYKA-c034 (N19A; tSH2 domains: aa 7–269) This paper N/A SYKA-c036 (full length) This paper N/A Software and algorithms DALI Holm and Sander24 http://ekhidna2.biocenter.helsinki.fi/dali/ DIALS Winter et al.36 https://dials.diamond.ac.uk AIMLESS Evans and Murshudov37 https://ccp4.ac.uk PHASER McCoy38 https://ccp4.ac.uk REFMAC5 Murshudov39 https://ccp4.ac.uk MolProbity Williams et al.40 http://molprobity.biochem.duke.edu PyMol (v2.2.2) Schrödinger, Inc. https://www.pymol.org/ DynDom Hayward and Berendsen41 https://dyndom.cmp.uea.ac.uk/dyndom/ PDBePISA Krissinel and Henrick42 https://www.ebi.ac.uk/pdbe/pisa/ ScÅtterIV – www.bioisis.net FIDA software v2.37 Fida Biosystems – HullRad v9 Fleming and Fleming43 http://52.14.70.9/ Microsoft Excel v2404 Microsoft https://www.microsoft.com/ GraphPad Prism v10.2.3 GraphPad Software https://www.graphpad.com/ Other Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S ..



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    175489 syka c024  (Addgene inc)
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    Addgene inc 175489 syka c024
    175489 Syka C024, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/175489+syka+c024/Myc-Foxo1+LAALL+(pCMV5)+(Plasmid+%2317548)/pm39442513-239-31-30
    Average 92 stars, based on 1 article reviews
    175489 syka c024 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

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